Optimization of Asymmetric PCR for Generation of a Single...

Aptamers are short DNA or RNA oligonucleotides with high, specific affinity to a special target. The name was originated from aptus that means to fit and meros that shows the polymer identity of oligonucleotides (1, 2). Aptamer characteristics provide prominent potential applications in multiple fields.These nucleic acid ligands are completely generated through in vitro process for a wide range of targets from small molecules and ions to large proteins and cells and even whole organism or tissue. Their chemical modifications could be easily performed to improve the intended specificity. Meanwhile, they keep their stability against various conditional stresses and show lower toxicity and immunogenicity than other specific ligands e.g. monoclonal antibodies. Because of the high specificity, adaptability, and ease of modification, aptamers have been used in a broad range of applications, including affinity purification, drug discovery, high-throughput screening, drug delivery, medical d iagnostics and biosensors (3-5). In molecular biology, there are several methods that could help researchers for in vitro evolution of single stranded oligonucleotide pools to high affinity ligands like aptamers. In vitro evolution is the experimental process in which large random-sequence pools of RNA or DNA are used as the starting point and particular nucleic acid sequences with higher affinity to an intended target are identified as aptamers. This type of selection and evolution is termed

Types Of Interviews From The University Career Center

There are many types of interviews and according to the UTSA University Career Center; there are over fourteen types of interviews ranging from individual interviews to group interviews to behavioral and clinical interviews. Dictionary.com defines interviewing as â€Å"a formal meeting in which one or more persons question, consult, or evaluate another person.† As a future Human Services professional interviewing will most likely be a major part of my career and since I am currently working with teens and young adults I have decided to focus and explore interviewing with adolescents. Adolescence is manifested by the onset of puberty â€Å"marked by dramatic changes in hormone levels and in physical appearance: physical growth, changes in facial structure, and appearance of secondary sexual characteristics. Over the same interval, adolescents experience numerous changes in social and other environmental influences, and typically enter a stage of profound psychological transiti on. The end of adolescence is said to occur when an individual has attained a stable adult role.† (Burnett, Blakemore, Dahl 2010, pg. 926). The onset of puberty can start as early as six years old and go as late as twenty-one years old. For many teens that is a period of trial and error, testing boundaries, values and establishing their own identities. For many it is a time where there body is awkward lengths, acne is barring its teeth and voice starts to crack at the worst times possible. The stage ofShow MoreRelatedCareer Journey For Success : My Career Path941 Words   |  4 Pages Career Journey to Success My career path was rerouted after the birth to my son. I was managing a 24-hour Alarm Monitoring Call Center with two years of college education in Psychology. Due to an inconsistent work schedule and difficulty locating Psychology courses that worked around my schedule, I decided to change my career path to Business Management, since I already had nearly 8 years experience in supervision and management. My mentor introduced me and made me familiarRead MoreAn Informational Interview: Sarah Buel1041 Words   |  4 PagesInformational Interview: Sarah Buel Because I am not certain what career path I intend to pursue, it was difficult to determine an appropriate person for my informational interview. However, following the advice at Quintessential Careers, I focused on my possible career choices (Quintessential Careers, Unk.). What I found was that I was likely to be involved in some type of work involving domestic violence, though I am still uncertain of the nature of that work. As a result, I decide to interview SarahRead MoreIs It Feasible To Increase The Number Of Students Who Attend799 Words   |  4 PagesIs it Feasible to Increase the number of students who attend CSU’s job fairs? Introduction The Career Center at Colorado State University is a vital part of a student’s time spent at the school. It provides students with opportunities to connect with people through a variety of networking channels. One way they help students meet potential employers, is through their job fairs. An increase in the amount of students who attend job fairs can directly increase the number of students who get jobsRead MoreCareer Counseling Is Crucial For College Students1271 Words   |  6 PagesCareer counseling is crucial for college students as they prepare for the real world. I have not been certified as a career counselor, but many aspects of my job require these types of conversations with my students. As a requirement for the career counseling class, I interviewed two student workers at the University of Cincinnati about their career aspirations and provided advice to help their success. The first student I interviewed had recently graduated college in May and was applying for teachingRead MoreAcademic Integration Into Career Technical Education1152 Words   |  5 Pagesinvestigate successful programs and curricula that implement academic integration into Career Technical Education programs at shared-time Career Technical Centers that lack teachers academically certified The constructivist paradigm emphasizes the importance of exploring how different stakeholders in a social setting construct their beliefs (Mertens, 2015). It also suggests that learners develop a theory and gain knowledge from their experiences; specifically, to understand a process or phenomenon (MertensRead MoreIdaho State University Resources Research Project1281 Words   |  6 PagesIdaho State University Resources Research Project Idaho State University has several fantastic qualities. Its Pharmacy Program is one of the best in the country and it has many other great programs for students to choose from. The problem for students is sometimes they do not know what major they should choose or career path they should follow. Idaho State has a number of helpful options for student who need to deiced on their major and pick a career path. These include the Career Center and StudentRead MoreCreating Your Career Plan By Henry Wadsworth873 Words   |  4 PagesCreating Your Career Plan Henry Wadsworth Longfellow penned one of my favorite sayings: â€Å"The heights by great men reached and kept, were not obtained by sudden flight, but they, while their companions slept, were toiling upward in the night† (Bill n.d.). My ninth grade English teacher had a new quote or adage for us to learn at the start of every class. I owe her for teaching me the definition of the word: Gumption, as well as introducing me to quotes that I have used to push myself when I thoughtRead MoreThe Job Advertisement That Make It Effective786 Words   |  4 Pagesmeeting with students each day to assist with career exploration and career planning. In addition, the position is a full-time opening, which helps the reader to understand they need to have certain qualifications before considering the position. The salary is presented below the overview so that the candidate can know the value of the position. During any job search, it can be frustrating to the candidate if the salary is not presented. When designing any type of position, it is best to be transparentRead MoreJournalism : An Objective And Ethical Way1218 Words   |  5 Pagesvariety of topics every day. A career in journalism is often a thankless and low paying job. The people who choose this job must have a passion for writing and informing the public. To further analyze a career in journalism, look at the steps to becoming one, the different types of journalism, and the qualities a journalist needs to become successful. There are a lot of steps to complete in the process of becoming a journalist. Many people begin preparing for a career in journalism by going to anRead MoreWorking My Pro Shop Assistant Job At Chisago Lakes Golf Course1189 Words   |  5 Pagesadvancing their brand. We see new shoes, new hats, new shirts come out of Nike every day. I believe I am the same way as Nike because I am always advancing myself in the latest, or newest ways to do things. Advancing as a person I believe sets you apart from the rest of the pack. I was listening to Dr. Eric Thomas, who is a motivational speaker out of Detroit. He said that if you learn something new every day for a year you learn 365 new things. If you learn something new every week that is 52 new things

Battle Of AP Back Essay Example For Students

Battle Of AP Back Essay On July 7, 1954 Bao Dai, president of Vietnam appointed Ding Diem asPrime Minister. Dai was a pro French president who did not have communistbeliefs for Vietnam. Diem was man who refused French rule and favored Americanideas. He was a Roman Catholic, thus causing him to be an opponent of communismas well. He also maintained a link to the CIA through Colonel Edward Lansdale,renowned expert on counterinsurgency. In April 1955 Diem launched an offensiveagainst his main rivals in the south (Coa Dai and Hoa Hoa sects well as thepowerful Binh Xuyen pirates) and declared himself president. Since the south wasa mainly Buddhist and Diem was Catholic, it caused him to be isolated from thepeople. His fear of a coup and obsession with power caused him to distrust all,but his own family. Between Diem and his family there was a spread of corruptionthroughout the country. By the late 1950s South Vietnam had degenerated intorepressive, undemocratic state, which left its people angry and isolated. 19 57sparked the first sign of trouble as guerillas launch attacks on governmentagencies in rural areas. It was believed that the Vietnamese Communist launchedthe attacks; also know as the Viet Cong (VC), who was of the Viet Ming partythat stayed behind. They had gone under ground a few years earlier in 1954. Theygained the support of North Vietnam and in 1959 began a policy to reunifyVietnam with a large-scale infiltration of armed cadres into the south along theHo Chi Man Trail. Due to the resentment caused by Diem, a substantial part ofSouth Vietnam was taken over by the communist (VC). The US, under the leadershipof President John F. Kennedy, was convinced that the insurgency in South Vietnamwas part of a Sino-Soviet campaign to ensure the spread of Communism. The USgovernment sent aid to South Vietnam in the form of US supplied M-113 armoredpersonnel carriers, helicopters, aircraft along with pilots and mechanics totrain South Vietnamese personnel on their use. Also US Special For ces (greenberets) and Army advisors were deployed to boost the capability of the Army ofthe Republic of Vietnam (ARVN). At the end of 1961, about 3,160 US servicepersonnel were in Vietnam. The number would rise to about 16,000 two yearslater. Even the advantages of US military technology and advisors to SouthVietnam, after a short time the VC learned to operate around them. This was dueto the AVRN unit commanders, many of who were just political appointees, lackedresolve. Thus allowing the VC to escape instead of risking heavy AVRNcasualties. The VC was engaging in new techniques ranging from the relocation ofvillagers to more fortified areas to use of air and artillery strikes in ruralareas. This resulted in more alienation of the people from Diems cause,ultimately leading to the peasant class assisting the VC. January 2, 1963 theAVRN 7th Division was ordered to destroy a VC in the hamlet (AP) of Tan Thoi. The plan was for AVRN infantry to be landed by helicopter to the north of thehamlet, while two Civil Guard battalions supported by a company of M-113sapproached from the south through the neighboring hamlet of Bac. Originally itwas believed that the transmitter was guarded by an estimate of 120 VC. Inreality it was guarded by about 360. The AVRN attack went wrong from the verystart. After the infantry had landed at 0703 hrs, it was discovered that theweather was not good enough for any more helicopter operations, due to a thickground fog. Further operations had to post-poned until 0930 hrs. The first clashoccurred at 0745 where the Civil Guard blundered into the VC section acrosstheir route. After the loss of their company commander the South Vietnamese Armywent to the ground and called for much needed reinforcements. Helicopters werelanded 300 yards west of Bac and were supported by UH-1 gunships. It is saidthat US pilots landed 200 yards from the hamlet into a VC ambush. As they ca mein at about 1020 hrs they were hit by machinegun and rifle fire from hiddenfoxholes. One of the CH-21 was shot down and another came in to recover theaircrew. That helicopter and a UH-1 met a similar fate. The infantry on boardtook cover in the paddy dikes. A call was made to the commander of the M-113sordering him to suggest an immediate advance on Bac, but the APCs were away tothe west beyond a series of canals. Some ARVN unit commanders had initiallyrefused to move. It was not until 1300 until the first APCs reached the battlearea. At 1430 hrs they charged the VC held positions, but the VC held theirground using grenades to hold off the vehicles. An airborne division was to themade available to the ARVN but were ordered the east of Bac to cut off theretreat of the VC. The VC was resourceful enough to wait until nightfall to slipaway into the darkness leaving only 18 dead behind. America suffered only 3fatalities, but the AVRN suffered over 80 due to the indecisiveness of theirl eaders. Later after much conflict in South Vietnam, a coup was plotted andcarried out against Diem. He was assassinated due to his oppressive natureagainst the Buddhist religion. America at this point could not back out of theconflict and was committed by November 1, 1963.